Journal: Biology of the Cell
Article Title: The Golgi apparatus in the endomembrane-rich gastric parietal cells exist as functional stable mini-stacks dispersed throughout the cytoplasm
doi: 10.1042/BC20110074
Figure Lengend Snippet: ( A , B ) Gastric cell populations were cultured for 24 h in gland culture medium containing ranitidine, then fixed in 1% formaldehyde, permeabilized and stained with either ( A ) TRITC-conjugated phalloidin, mouse anti-GM130 ( cis -Golgi marker) antibodies followed by FITC-conjugated anti-mouse IgG and rabbit anti-syntaxin 16 (TGN marker) antibodies followed by Alexa Fluor® 647-conjugated to anti-rabbit IgG or ( B ) mouse anti-HKα antibodies followed by FITC-conjugated anti-mouse IgG, rabbit antiGCC185 (TGN marker) antibodies followed by Alexa Fluor® 647-conjugated anti-rabbit IgG (pseudo-coloured green) and human anti-p230 (TGN marker) antibodies followed by Alexa Fluor® 594-conjugated to anti-human IgG. ( C ) Gastric glands were isolated from the stomachs of wild-type mice (fasted) and fixed in 4% PFA, permeabilized and stained with TRITC-conjugated phalloidin, mouse anti-GM130 antibodies followed by FITC-conjugated anti-mouse IgG and rabbit anti-syntaxin16 (TGN marker) antibodies followed by Alexa Fluor® 647-conjugated to anti-rabbit IgG. Scale bars represent 10 μm.
Article Snippet: A rabbit polyclonal antibody against syntaxin 16 was purchased from Synaptic Systems (Gottingen, Germany).
Techniques: Cell Culture, Staining, Marker, Isolation